Genotyping
The Translational Genomics Core at Partners Personalized Medicine provides flexible, high-quality, high-throughput SNP genotyping to the Partners research community. We offer Taqman genotyping, as well as human genome-wide methylation analysis, and Infinium assays on our Illumina iSCAN platform. The Partners Personalized Medicine Translational Genomics Core genotyping facility employs state-of-the-art equipment, including Biomek FX, Perkin Elmer Janus, a 384-well ABI Prism 7900 HT Sequence Detection System, ABI 7500 Sequence Detection System, Biotrove OpenArray SNP Genotyping Platform and an Illumina iScan system.
| Technology | Project Size | SNP Info | DNA | Concentration | Quantitation | Minimum Quantity |
| Illumina Infinium | up to 5M SNPs | Fixed Panels | Genomic DNA Only | 50 ng/ul | Picogreen | 20 ul |
| Taqman | 1-8 SNPs | RS# or customer provided sequence information | Genomic or WGA DNA | 5-10 ng/ul | Picogreen | 30 ul |
| OpenArray | 16, 32, 64, 128, or 256 SNPs | RS# or customer provided sequence information | Genomic or WGA DNA | 50 ng/ul | Picogreen |
20 ul |
| Methylation | Samples in multiples of 24 | N/A | Genomic DNA or Bisulfite-Converted DNA | 50 ng/ul of Genomic DNA | Picogreen | 2 ug |
