The Translational Genomics Core at Partners Personalized Medicine provides flexible, high-quality, high-throughput SNP genotyping to the Partners research community. We offer Taqman genotyping, as well as human genome-wide methylation analysis, custom and standard panel GoldenGate and Infinium assays on our Illumina iSCAN platform. The Partners Personalized Medicine Translational Genomics Core genotyping facility employs state-of-the-art equipment, including Biomek FX, Perkin Elmer Janus, a 384-well ABI Prism 7900 HT Sequence Detection System, ABI 7500 Sequence Detection System, Biotrove OpenArray SNP Genotyping Platform and an Illumina iScan system.
|Technology||Project Size||SNP Info||DNA||Concentration||Quantitation||Minimum Quantity|
|Illumina GoldenGate||384-1536 SNPs in multiples of 96 SNPs||Custom or Fixed||Genomic or WGA DNA||50 ng/ul||Picogreen||15 ul|
|Illumina Infinium||up to 5M SNPs||Fixed Panels, Custom Additions, Added Exome Content||Genomic DNA Only||50 ng/ul||Picogreen||20 ul|
|Taqman||1-8 SNPs||RS# or customer provided sequence information||Genomic or WGA DNA||5-10 ng/ul||Picogreen||30 ul|
|OpenArray||16, 32, 64, 128, or 256 SNPs||RS# or customer provided sequence information||Genomic or WGA DNA||50 ng/ul||Picogreen||
|Methylation||Samples in multiples of 24||N/A||Genomic DNA or Bisulfite-Converted DNA||50 ng/ul of Genomic DNA||Picogreen||2 ug|