The Partners Biobank offers the following biobanking and sample processing services:
DNA Extraction from Whole Blood and Saliva
We are able to accept fresh or frozen whole blood for DNA extraction. Frozen blood or blood that has been held in cold storage for greater than five days post draw will be processed under a special protocol to enhance yields. Samples of extracted DNA are subject to QC by 260/280 OD ratio and absorbance at 260nm, as well as picogreen quantification.
- Blood – 5-10mL whole blood in Purple top (K3EDTA) vacutainer vial.
- Saliva – For best results collect in Oragene kit.
Reporting of Results
Extractions will be completed within 5 business days upon receipt of sample. All data will be available via GIGPAD. Investigators (or those listed as contacts) will have access to reports that will include the sample ID entered by the research team, the Partners Biobank facility ID, the status of the sample, the concentration, and volume.
RNA Extraction from Whole Blood with PaxGene Tubes
We do not provide the PaxGene Blood Collection tubes. These may be purchased from Qiagen. We will take the PaxGene tubes submitted to us for processing and provide the service to extract RNA, provide a Bioanalyzer QC check for the sample and 260nm absorbance concentration reading. Extracted RNA samples are stored in 0.5ml tubes at -80°C immediately after the RNA is QC'd.
Each PaxGene Blood tube will hold 5 ml of blood and on average yields about 5 ug of RNA from a properly collected tube. According to the manufacturer's data: "Blood RNA Tubes are intended for the collection of whole blood and stabilization of cellular RNA for up to three days at 18-25°C or up to five days at 2-8°C. Currently available data shows stabilization of cellular RNA for up to 8 years at –20°C or -70°C".
IMPORTANT: Once chilled or frozen the PaxGene Tube must be transported in a manner to maintain temperature and not be warmed. Failure to ensure tubes maintain temperature after storage at 4°C or freezing during handling and transport will adversely affect RNA yield and integrity.
Blood – 5mL whole blood in Paxgene Tube (manufacturer Qiagen)
Reporting of Results
Extractions will be completed within 10 business days upon receipt of sample. All data will be available via GIGPAD. Investigators (or those listed as contacts) will have access to reports that will include the sample ID entered by the research team, the Partners Biobank facility ID, the status of the sample, the concentration, and volume.
Lymphoblast transformation services are performed using Epstein-Barr virus transformations on whole blood samples for the initiation of lymphoblastoid cell lines. Blood samples should be sent to Partners Personalized Medicine – Partners Biobank (65 Landsdowne Street, Cambridge, MA 02139) via overnight or same day courier. Bloods designated for transformation on the same day are only accepted Monday through Friday between the hours of 9:00 a.m. and 2:30 p.m. Shipping Detail.
For best results, samples need to be received no later than 72 hours after being drawn. Samples received after 72 hours will be processed with the understanding that if the transformation is not successful, payment will still be required for the service. Samples should be kept at room temperature at all times and never refrigerated. For samples received within the requested 72 hours from blood draw, the Core has a success rate of greater than 90%. However, occasional failure to transform is inherent to this process and is to be expected.
No cell lines from cultures maintained elsewhere and sent to us for storage may be grown in our incubators unless they have been first subjected to quarantine and mycoplasma testing and shown to be free of contamination.
Whole blood collected in a 10mL “Yellow top” ACD vacutainer vial and maintained at ROOM TEMPERATURE. DO NOT CHILL.
Reporting of results
Successful transformations are usually evident within 4-6 weeks upon macroscopic examination of the culture. All data will be available via GIGPAD. Investigators (or those listed as contacts) will have access to reports that will include the sample ID entered by the research team, the Partners Biobank facility ID, and the status of the sample.
New Culture, less than ~4 weeks will not have any information. Please contact lab directly for information pertaining to specific specimens.
Approximately 2-4ml of plasma or serum will be isolated from each 10ml tube of blood. Samples delivered to our lab are processed as quickly as possible upon arrival and completion of accessioning. Plasma/Serum isolates will be aliquoted in 1ml volume and stored in –80°C freezers.
- Plasma – 10mL whole blood in “Purple top” (K3EDTA) vacutainer vial.
- Serum – 10mL whole blood in “Red top” (no anticoagulant) vacutainer vial.
Reporting of Results
Plasma isolations will be completed within 1 business day upon receipt of sample. All data will be available via GIGPAD. Investigators (or those listed as contacts) will have access to reports that will include the sample ID entered by the research team, the Partners Biobank facility ID, and the status of the sample.
Samples will be arrayed into a 96 well plate format using an automated liquid handling robot and normalized to the requested concentration. The first plate of each array created is considered a "Master Plate" (94 samples/plate). All partial plates (<94 samples) will be charged the price of a full plate. We will visually inspect the plates to ensure volume is present in each well. The plate record will be made available to the researcher (or those listed as contacts) in Excel format along with the sample IDs provided by the researcher, and final concentrations quantified by picogreen
Sample Storage and Retrieval
We can retrieve samples from storage from your supplied requests via Gateway for Integration of Genomic-Proteomic Applications and Data (GIGPAD).
*Sample storage fees are applied on a quarterly basis.
Please contact Sarah Yuhas (firstname.lastname@example.org) for additional information.